Activation of NF-κB/p65 facilitates early chondrogenic differentiation during endochondral ossification.

ABSTRACT:

Background

NF-κB/p65 has been reported to be involved in regulation of chondrogenic differentiation. However, its function in relation to key chondrogenic factor Sox9 and onset of chondrogenesis during endochondral ossification is poorly understood. We hypothesized that the early onset of chondrogenic differentiation is initiated by transient NF-κB/p65 signaling.

Methodology/principal findings

The role of NF-κB/p65 in early chondrogenesis was investigated in different in vitro, ex vivo and in vivo endochondral models: ATDC5 cells, hBMSCs, chicken periosteal explants and growth plates of 6 weeks old mice. NF-κB/p65 activation was manipulated using pharmacological inhibitors, RNAi and activating agents. Gene expression and protein expression analysis, and (immuno)histochemical

SUBMITTER: Caron MM 

PROVIDER: S-EPMC3299787 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature