Maternal Mthfd1 disruption impairs fetal growth but does not cause neural tube defects in mice.

ABSTRACT:

Background

MTHFD1 encodes C1-tetrahydrofolate synthase, which is a folate-dependent enzyme that catalyzes the formation and interconversion of folate-activated one-carbon groups for nucleotide biosynthesis and cellular methylation. A polymorphism in MTHFD1 (1958G→A) impairs enzymatic activity and is associated with increased risk of adverse pregnancy outcomes, but the mechanisms are unknown.

Objective

The objective of this study was to determine whether disruption of the embryonic or maternal Mthfd1 gene or both interacts with impaired folate and choline status to affect neural tube closure, fetal growth, and fertility in mice and to investigate the underlying metabolic disruptions.

Design

Dams with a gene-trapped (gt) allele in Mthfd1 and wild-type dams were fed a co

SUBMITTER: Beaudin AE 

PROVIDER: S-EPMC3302363 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature