A novel approach for the rapid mutagenesis and directed evolution of the structural genes of west nile virus.

ABSTRACT: Molecular clone technology has proven to be a powerful tool for investigating the life cycle of flaviviruses, their interactions with the host, and vaccine development. Despite the demonstrated utility of existing molecular clone strategies, the feasibility of employing these existing approaches in large-scale mutagenesis studies is limited by the technical challenges of manipulating relatively large molecular clone plasmids that can be quite unstable when propagated in bacteria. We have developed a novel strategy that provides an extremely rapid approach for the introduction of mutations into the structural genes of West Nile virus (WNV). The backbone of this technology is a truncated form of the genome into which DNA fragments harboring the structural genes are ligated and transfected di

SUBMITTER: Lin TY 

PROVIDER: S-EPMC3302547 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature