A tightly controlled conditional knockdown system using the Tol2 transposon-mediated technique.

ABSTRACT:

Background

Gene knockdown analyses using the in utero electroporation method have helped reveal functional aspects of genes of interest in cortical development. However, the application of this method to analyses in later stages of brain development or in the adult brain is still difficult because the amount of injected plasmids in a cell decreases along with development due to dilution by cell proliferation and the degradation of the plasmids. Furthermore, it is difficult to exclude the influence of earlier knockdown effects.

Methodology/principal findings

We developed a tightly controlled conditional knockdown system using a newly constructed vector, pT2K-TBI-shRNAmir, based on a Tol2 transposon-mediated gene transfer methodology with the tetracycline-inducible gene expres

SUBMITTER: Iguchi T 

PROVIDER: S-EPMC3302819 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature