Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology.

ABSTRACT: The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integrated computational approach to characterize novel human spindle components and have analysed in detail the individual candidates predicted to be spindle proteins, as well as the network of predicted relations connecting known and putative spindle proteins. The subsequent experimental validation of a number of predicted novel proteins confirmed not only their association with the spindle apparatus but

SUBMITTER: Rojas AM 

PROVIDER: S-EPMC3302876 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature