Strain-specific modifier genes governing craniofacial phenotypes.

ABSTRACT:

Background

The presence of strain-specific modifier genes is known to modulate the phenotype and pathophysiology of mice harboring genetically engineered mutations. Thus, identification of genetic modifier genes is requisite to understanding control of phenotypic expression. c-Ski is a transcriptional regulator. Ski(-/-) mice on a C57BL6J (B6) background exhibit facial clefting, while Ski(-/-) mice on a 129P3 (129) background present with exencephaly.

Methods

In the present study, oligonucleotide-based gene expression profiling was used to identify potential strain-specific modifier gene candidates present in wild type mice of B6 and 129 genetic backgrounds. Changes in gene expression were verified by TaqMan quantitative real-time PCR.

Results

Steady-state levels of 8

SUBMITTER: Mukhopadhyay P 

PROVIDER: S-EPMC3302963 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature