Identification of conserved splicing motifs in mutually exclusive exons of 15 insect species.

ABSTRACT:

Background

During alternative splicing, the inclusion of an exon in the final mRNA molecule is determined by nuclear proteins that bind cis-regulatory sequences in a target pre-mRNA molecule. A recent study suggested that the regulatory codes of individual RNA-binding proteins may be nearly immutable between very diverse species such as mammals and insects. The model system Drosophila melanogaster therefore presents an excellent opportunity for the study of alternative splicing due to the availability of quality EST annotations in FlyBase.

Methods

In this paper, we describe an in silico analysis pipeline to extract putative exonic splicing regulatory sequences from a multiple alignment of 15 species of insects. Our method, ESTs-to-ESRs (E2E), uses graph analysis of EST splic

SUBMITTER: Buendia P 

PROVIDER: S-EPMC3303723 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature