Chemotherapy-induced toxicity is highly heritable in Drosophila melanogaster.

ABSTRACT:

Objectives

Identification of the genes responsible for chemotherapy toxicity in Drosophila melanogaster may allow for the identification of human orthologs that similarly mediate toxicity in humans. To develop D. melanogaster as a model of dissecting chemotoxicity, we first need to develop standardized high-throughput toxicity assays and prove that the interindividual variation in toxicity as measured by such assays is highly heritable.

Methods

We developed a method for the oral delivery of commonly used chemotherapy drugs to Drosophila. Post-treatment female fecundity displayed a dose-dependent response to varying levels of the chemotherapy drug delivered. We fixed the dose for each drug at a level that resulted in a 50% reduction in fecundity and used a paternal half-sibli

SUBMITTER: Kislukhin G 

PROVIDER: S-EPMC3303944 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature