Upregulation of the ERG11 gene in Candida krusei by azoles.

ABSTRACT:

Background and the purpose of the study

Candida species are the agents of local and systemic opportunistic infections and have become a major cause of morbidity and mortality in the last few decades. Azole resistance in Candida krusei (C. krusei) species appears to be the result of gene alterations in relation to the ergosterol biosynthesis pathway, as well as efflux pumps. The main objective of this study was to examine the RNA expression of ERG11 in C. krusei which had been identified to be resistance to azoles.

Methods

The ERG11 mRNA expression was investigated in four Iranian clinical isolates of C. krusei, which were resistant to fluconazole and itraconazole by a semiquantitative RT-PCR.

Results

The mRNA expression levels were observed in all four isolates by thi

SUBMITTER: Tavakoli M 

PROVIDER: S-EPMC3304355 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature