The presence of amorpha-4, 11-diene synthase, a key enzyme in artemisinin production in ten Artemisia species.

ABSTRACT:

Background and the purpose of the study

Artemisinin is one of the most effective medicine against malaria, which is produced naturally by Artemisia annua in low yield. It is produced in a metabolic pathway, in which several genes and gene products are involved. One of the key genes in this pathway is am1, which encodes amorpha-4, 11-diene synthase (ADS), a key enzyme in artemisinin biosynthesis pathway. The aim of this study was to determine the presence of this gene in ten Artemisia species in order to increase the yield of production of Artemisinin.

Methods

The experiments were carried out using PCR. Specific primers were designed based on the published am1 gene sequence obtained from A. annua (NCBI, accession number AF327527).

Results

The amplification of this gene

SUBMITTER: Hosseini R 

PROVIDER: S-EPMC3304396 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature