Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria.

ABSTRACT:

Background

Overexpression of plasma membrane multi-drug resistance protein 1 (MRP-1) can lead to multidrug resistance. In this study, we describe for the first time the expression of mitochondrial MRP-1 in untreated human normal and cancer cells and tissues.

Methods

MRP-1 expression and subcellular localisation in normal and cancer cells and tissues was examined by differential centrifugation and western blotting, and immunofluorescence microscopy. Viable mitochondria were isolated and MRP-1 efflux activity measured using the calcein-AM functional assay. MRP-1 expression was increased using retroviral infection and specific overexpression confirmed by RNA array. Cell viability was determined by trypan blue exclusion and annexin V-propidium iodide labelling of cells.

Resul

SUBMITTER: Roundhill EA 

PROVIDER: S-EPMC3304412 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature