Dissection of stromal and cancer cell-derived signals in melanoma xenografts before and after treatment with DMXAA.

ABSTRACT:

Background

The non-malignant cells of the tumour stroma have a critical role in tumour biology. Studies dissecting the interplay between cancer cells and stromal cells are required to further our understanding of tumour progression and methods of intervention. For proof-of-principle of a multi-modal approach to dissect the differential effects of treatment on cancer cells and stromal cells, we analysed the effects of the stromal-targeting agent 5,6-dimethylxanthenone-4-acetic acid on melanoma xenografts.

Methods

Flow cytometry and multi-colour immunofluorescence staining was used to analyse leukocyte numbers in xenografts. Murine-specific and human-specific multiplex cytokine panels were used to quantitate cytokines produced by stromal and melanoma cells, respectively. Human

SUBMITTER: Henare K 

PROVIDER: S-EPMC3304430 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature