Highly sensitive and specific detection of rare variants in mixed viral populations from massively parallel sequence data.

ABSTRACT: Viruses diversify over time within hosts, often undercutting the effectiveness of host defenses and therapeutic interventions. To design successful vaccines and therapeutics, it is critical to better understand viral diversification, including comprehensively characterizing the genetic variants in viral intra-host populations and modeling changes from transmission through the course of infection. Massively parallel sequencing technologies can overcome the cost constraints of older sequencing methods and obtain the high sequence coverage needed to detect rare genetic variants (< 1%) within an infected host, and to assay variants without prior knowledge. Critical to interpreting deep sequence data sets is the ability to distinguish biological variants from process errors with high sensitivit

SUBMITTER: Macalalad AR 

PROVIDER: S-EPMC3305335 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature