Ruminal microbe of biohydrogenation of trans-vaccenic acid to stearic acid in vitro.

ABSTRACT:

Background

Optimization of the unsaturated fatty acid composition of ruminant milk and meat is desirable. Alteration of the milk and fatty acid profile was previously attempted by the management of ruminal microbial biohydrogenation. The aim of this study was to identify the group of ruminal trans-vaccenic acid (trans-11 C18:1, t-VA) hydrogenating bacteria by combining enrichment studies in vitro.

Methods

The enrichment culture growing on t-VA was obtained by successive transfers in medium containing t-VA. Fatty acids were detected by gas chromatograph and changes in the microbial composition during enrichment were analyzed by denaturing gradient gel electrophoresis (DGGE). Prominent DGGE bands of the enrichment cultures were identified by 16S rRNA gene sequencing.

Result

SUBMITTER: Li D 

PROVIDER: S-EPMC3305423 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature