A systematic analysis of cell cycle regulators in yeast reveals that most factors act independently of cell size to control initiation of division.

ABSTRACT: Upstream events that trigger initiation of cell division, at a point called START in yeast, determine the overall rates of cell proliferation. The identity and complete sequence of those events remain unknown. Previous studies relied mainly on cell size changes to identify systematically genes required for the timely completion of START. Here, we evaluated panels of non-essential single gene deletion strains for altered DNA content by flow cytometry. This analysis revealed that most gene deletions that altered cell cycle progression did not change cell size. Our results highlight a strong requirement for ribosomal biogenesis and protein synthesis for initiation of cell division. We also identified numerous factors that have not been previously implicated in cell cycle control mechanisms. W

SUBMITTER: Hoose SA 

PROVIDER: S-EPMC3305459 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature