Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum).

ABSTRACT:

Background

MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified. Wounding is believed to induce defensive response in tobacco, but the mechanism responsible for this response is yet to be uncovered.

Results

To get insight into the role of small RNAs in damage-induced responses, we sequenced and analysed small RNA populations in roots and leaves from woundi

SUBMITTER: Tang S 

PROVIDER: S-EPMC3306195 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature