A novel high-throughput vaccinia virus neutralization assay and preexisting immunity in populations from different geographic regions in China.

ABSTRACT:

Background

Pre-existing immunity to Vaccinia Tian Tan virus (VTT) resulting from a large vaccination campaign against smallpox prior to the early 1980s in China, has been a major issue for application of VTT-vector based vaccines. It is essential to establish a sensitive and high-throughput neutralization assay to understand the epidemiology of Vaccinia-specific immunity in current populations in China.

Methodology/principal findings

A new anti-Vaccinia virus (VACV) neutralization assay that used the attenuated replication-competent VTT carrying the firefly luciferase gene of Photinus pyralis (rTV-Fluc) was established and standardized for critical parameters that included the choice of cell line, viral infection dose, and the infection time. The current study evaluated the

SUBMITTER: Liu Q 

PROVIDER: S-EPMC3306400 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature