Novel features of ARS selection in budding yeast Lachancea kluyveri.

ABSTRACT:

Background

The characterization of DNA replication origins in yeast has shed much light on the mechanisms of initiation of DNA replication. However, very little is known about the evolution of origins or the evolution of mechanisms through which origins are recognized by the initiation machinery. This lack of understanding is largely due to the vast evolutionary distances between model organisms in which origins have been examined.

Results

In this study we have isolated and characterized autonomously replicating sequences (ARSs) in Lachancea kluyveri - a pre-whole genome duplication (WGD) budding yeast. Through a combination of experimental work and rigorous computational analysis, we show that L. kluyveri ARSs require a sequence that is similar but much longer than the ARS

SUBMITTER: Liachko I 

PROVIDER: S-EPMC3306766 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature