A direct detection of Escherichia coli genomic DNA using gold nanoprobes.

ABSTRACT:

Background

In situation like diagnosis of clinical and forensic samples there exists a need for highly sensitive, rapid and specific DNA detection methods. Though conventional DNA amplification using PCR can provide fast results, it is not widely practised in diagnostic laboratories partially because it requires skilled personnel and expensive equipment. To overcome these limitations nanoparticles have been explored as signalling probes for ultrasensitive DNA detection that can be used in field applications. Among the nanomaterials, gold nanoparticles (AuNPs) have been extensively used mainly because of its optical property and ability to get functionalized with a variety of biomolecules.

Results

We report a protocol for the use of gold nanoparticles functionalized with sing

SUBMITTER: Padmavathy B 

PROVIDER: S-EPMC3306828 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature