Full field optical coherence tomography can identify spermatogenesis in a rodent sertoli-cell only model.

ABSTRACT:

Background

Microdissection testicular sperm extraction (micro-TESE) has replaced conventional testis biopsies as a method of choice for obtaining sperm for in vitro fertilization for men with nonobstructive azoospermia. A technical challenge of micro-TESE is that the low magnification inspection of the tubules with a surgical microscope is insufficient to definitively identify sperm-containing tubules, necessitating tissue removal and cytologic assessment. Full field optical coherence tomography (FFOCT) uses white light interference microscopy to generate quick high-resolution tomographic images of fresh (unprocessed and unstained) tissue. Furthermore, by using a nonlaser safe light source (150 W halogen lamp) for tissue illumination, it ensures that the sperm extracted for in vitr

SUBMITTER: Ramasamy R 

PROVIDER: S-EPMC3307228 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature