Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current.

ABSTRACT:

Aims

Remodelling and regional gradients in expression of connexins (Cx) are thought to contribute to atrial electrical dysfunction and atrial fibrillation. We assessed the effect of interaction between Cx43, Cx40, and Cx45 on atrial cell-to-cell coupling and inward Na current (I(Na)) in engineered pairs of atrial myocytes derived from wild-type mice (Cx43(+/+)) and mice with genetic ablation of Cx43 (Cx43(-/-)).

Methods and results

Cell pairs were engineered by microcontact printing from atrial Cx43(+/+) and Cx43(-/-) murine myocytes (1 day before birth, 3-5 days in culture). Dual and single voltage clamp were used to measure intercellular electrical conductance, g(j), and its dependence on transjunctional voltage, V(j), single gap junction channel conductances, and I(Na). 3

SUBMITTER: Desplantez T 

PROVIDER: S-EPMC3307380 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature