Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.

ABSTRACT: Human induced pluripotent stem cells (hiPSC) hold great promise for providing various differentiated cell models for in vitro toxigenicity testing. For Clostridium botulinum neurotoxin (BoNT) detection and mechanistic studies, several cell models currently exist, but none examine toxin function with species-specific relevance while exhibiting high sensitivity. The most sensitive cell models to date are mouse or rat primary cells and neurons derived from mouse embryonic stem cells, both of which require significant technical expertise for culture preparation. This study describes for the first time the use of hiPSC-derived neurons for BoNT detection. The neurons used in this study were differentiated and cryopreserved by Cellular Dynamics International (Madison, WI) and consist of an almost

SUBMITTER: Whitemarsh RC 

PROVIDER: S-EPMC3307606 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature