A comparative analysis of exome capture.

ABSTRACT:

Background

Human exome resequencing using commercial target capture kits has been and is being used for sequencing large numbers of individuals to search for variants associated with various human diseases. We rigorously evaluated the capabilities of two solution exome capture kits. These analyses help clarify the strengths and limitations of those data as well as systematically identify variables that should be considered in the use of those data.

Results

Each exome kit performed well at capturing the targets they were designed to capture, which mainly corresponds to the consensus coding sequences (CCDS) annotations of the human genome. In addition, based on their respective targets, each capture kit coupled with high coverage Illumina sequencing produced highly accurate nu

SUBMITTER: Parla JS 

PROVIDER: S-EPMC3308060 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature