In situ hybridization for the precise localization of transcripts in plants.

ABSTRACT: With the advances in genomics research of the past decade, plant biology has seen numerous studies presenting large-scale quantitative analyses of gene expression. Microarray and next generation sequencing approaches are being used to investigate developmental, physiological and stress response processes, dissect epigenetic and small RNA pathways, and build large gene regulatory networks(1-3). While these techniques facilitate the simultaneous analysis of large gene sets, they typically provide a very limited spatiotemporal resolution of gene expression changes. This limitation can be partially overcome by using either profiling method in conjunction with lasermicrodissection or fluorescence-activated cell sorting(4-7). However, to fully understand the biological role of a gene, knowledge

SUBMITTER: Javelle M 

PROVIDER: S-EPMC3308598 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature