Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions.

ABSTRACT: The prototypical representatives of the Euryarchaeota--the methanogens--are oxygen sensitive and are thought to occur only in highly reduced, anoxic environments. However, we found methanogens of the genera Methanosarcina and Methanocella to be present in many types of upland soils (including dryland soils) sampled globally. These methanogens could be readily activated by incubating the soils as slurry under anoxic conditions, as seen by rapid methane production within a few weeks, without any additional carbon source. Analysis of the archaeal 16S ribosomal RNA gene community profile in the incubated samples through terminal restriction fragment length polymorphism and quantification through quantitative PCR indicated dominance of Methanosarcina, whose gene copy numbers also correlated wit

SUBMITTER: Angel R 

PROVIDER: S-EPMC3309352 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature