Negative feedback confers mutational robustness in yeast transcription factor regulation.

ABSTRACT: Organismal fitness depends on the ability of gene networks to function robustly in the face of environmental and genetic perturbations. Understanding the mechanisms of this stability is one of the key aims of modern systems biology. Dissecting the basis of robustness to mutation has proven a particular challenge, with most experimental models relying on artificial DNA sequence variants engineered in the laboratory. In this work, we hypothesized that negative regulatory feedback could stabilize gene expression against the disruptions that arise from natural genetic variation. We screened yeast transcription factors for feedback and used the results to establish ROX1 (Repressor of hypOXia) as a model system for the study of feedback in circuit behaviors and its impact across genetically hete

SUBMITTER: Denby CM 

PROVIDER: S-EPMC3309721 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature