Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers.

ABSTRACT:

Aim

To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.

Methods

We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.

Results

We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 wer

SUBMITTER: Kocevar N 

PROVIDER: S-EPMC3309911 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature