Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production.

ABSTRACT:

Background

Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of wax esters that are high-value materials and can be used in a variety of industrial applications. The products of WSs include fatty acid ethyl esters, which can be directly used as biodiesel.

Results

Here, heterologous WSs derived from five different organisms were successfully expressed and evaluated for their substrate preference in Saccharomyces cerevisiae. We investigated the potential of the different WSs for biodiesel (that is, fatty acid ethyl esters) production in S. cerevisiae. All investigated WSs, from Acinetobacter baylyi ADP1, Marinobacter hydro

SUBMITTER: Shi S 

PROVIDER: S-EPMC3309958 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature