DnaA and the timing of chromosome replication in Escherichia coli as a function of growth rate.

ABSTRACT:

Background

In Escherichia coli, overlapping rounds of DNA replication allow the bacteria to double in faster times than the time required to copy the genome. The precise timing of initiation of DNA replication is determined by a regulatory circuit that depends on the binding of a critical number of ATP-bound DnaA proteins at the origin of replication, resulting in the melting of the DNA and the assembly of the replication complex. The synthesis of DnaA in the cell is controlled by a growth-rate dependent, negatively autoregulated gene found near the origin of replication. Both the regulatory and initiation activity of DnaA depend on its nucleotide bound state and its availability.

Results

In order to investigate the contributions of the different regulatory processes to the

SUBMITTER: Grant MA 

PROVIDER: S-EPMC3309966 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature