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Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.

ABSTRACT: Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X-ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 72.5, b = 136.8, c = 273.3 Å, suggesting the presence of one complex in the asymmetric unit.

SUBMITTER: Maruyama Y 

PROVIDER: S-EPMC3310540 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.

Maruyama Yukie Y   Itoh Takafumi T   Nishitani Yu Y   Mikami Bunzo B   Hashimoto Wataru W   Murata Kousaku K  

Acta crystallographica. Section F, Structural biology and crystallization communications 20120223 Pt 3

Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Preliminary X-ray analysis showed that the crystal diffracted to 3.3 Å resolution and belonged to space group  ...[more]

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