Electrical pulse stimulation of cultured human skeletal muscle cells as an in vitro model of exercise.

ABSTRACT:

Background and aims

Physical exercise leads to substantial adaptive responses in skeletal muscles and plays a central role in a healthy life style. Since exercise induces major systemic responses, underlying cellular mechanisms are difficult to study in vivo. It was therefore desirable to develop an in vitro model that would resemble training in cultured human myotubes.

Methods

Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose and oleic acid metabolism were studied using radio-labeled substrates, and gene expression was analyzed using real-time RT-PCR. Mitochondrial content and function were measured by live imaging and determination of citrate synthase activity, res

SUBMITTER: Nikolic N 

PROVIDER: S-EPMC3310863 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature