Project description:We developed an artificial signaling system to enable the assembly of cytokine receptor surrogate ligands. A JAK-STAT5 signaling module—comprising the JAK3-specific γc receptor and a functional IL-2Rβ receptor—was stably expressed in a STAT-deficient cell line. Using a combinatorial antibody library, we screened for receptor binders with selective emphasis on IL-2Rβ affinity. Among various bispecific scaffolds, the single-chain diabody (scDb) format demonstrated superior potency and efficacy, effectively recapitulating native IL-2 ligand activity by inducing functional dimerization of IL-2Rβ and γc receptors. The lead scDb-2d (also termed J9H4) agonist antibody functioned as a surrogate IL-2 receptor ligand, selectively driving Teff and NK cell proliferation and activation.

2025-12-31 | GSE305574 | GEO

Misfolded proinsulin impairs processing of precursor of insulin receptor and insulin signaling in β cells.

Project description: Not available

| S-EPMC6766638 | biostudies-literature

Chemically Precise Glycoengineering Improves Human Insulin.

Project description: Not available

| S-EPMC6287623 | biostudies-literature

Proinsulin folding and trafficking defects trigger a common pathological disturbance of endoplasmic reticulum homeostasis.

Project description: Not available

| S-EPMC10955614 | biostudies-literature