Versatile pathway-centric approach based on high-throughput sequencing to anticancer drug discovery.

ABSTRACT: The advent of powerful genomics technologies has uncovered many fundamental aspects of biology, including the mechanisms of cancer; however, it has not been appropriately matched by the development of global approaches to discover new medicines against human diseases. Here we describe a unique high-throughput screening strategy by high-throughput sequencing, referred to as HTS(2), to meet this challenge. This technology enables large-scale and quantitative analysis of gene matrices associated with specific disease phenotypes, therefore allowing screening for small molecules that can specifically intervene with disease-linked gene-expression events. By initially applying this multitarget strategy to the pressing problem of hormone-refractory prostate cancer, which tends to be accelerated by

SUBMITTER: Li H 

PROVIDER: S-EPMC3311343 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature