Coffee cysteine proteinases and related inhibitors with high expression during grain maturation and germination.

ABSTRACT:

Background

Cysteine proteinases perform multiple functions in seeds, including participation in remodelling polypeptides and recycling amino acids during maturation and germination. Currently, few details exist concerning these genes and proteins in coffee. Furthermore, there is limited information on the cysteine proteinase inhibitors which influence the activities of these proteinases.

Results

Two cysteine proteinase (CP) and four cysteine proteinase inhibitor (CPI) gene sequences have been identified in coffee with significant expression during the maturation and germination of coffee grain. Detailed expression analysis of the cysteine proteinase genes CcCP1 and CcCP4 in Robusta using quantitative RT-PCR showed that these transcripts accumulate primarily during grain matu

SUBMITTER: Lepelley M 

PROVIDER: S-EPMC3311568 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature