Potential of exogenous cartilage proteoglycan as a new material for cartilage regeneration.

ABSTRACT:

Background

Although proteoglycan (PG) is one of the major components of cartilage matrices, its biological function is not fully elucidated.

Methods

The objectives of this study were to investigate the proliferation and differentiation of chondrocytes embedded in atelocollagen gel with exogenous cartilage PG (PG-atelocollagen gel) in vitro, and also to evaluate the repair of cartilage defects by PG-atelocollagen gel in vivo. In the in vitro study, rabbit chondrocytes were cultured in the PG-atelocollagen gel. Cell proliferation and mRNA expression levels were measured, and gels were histologically evaluated. In the in vivo study, cultured PG-atelocollagen gel containing chondrocytes were transplanted into full-thickness articular cartilage defects in rabbit knees, and evalua

SUBMITTER: Ohshika S 

PROVIDER: S-EPMC3311805 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature