Computer-based annotation of putative AraC/XylS-family transcription factors of known structure but unknown function.

ABSTRACT: Currently, about 20 crystal structures per day are released and deposited in the Protein Data Bank. A significant fraction of these structures is produced by research groups associated with the structural genomics consortium. The biological function of many of these proteins is generally unknown or not validated by experiment. Therefore, a growing need for functional prediction of protein structures has emerged. Here we present an integrated bioinformatics method that combines sequence-based relationships and three-dimensional (3D) structural similarity of transcriptional regulators with computer prediction of their cognate DNA binding sequences. We applied this method to the AraC/XylS family of transcription factors, which is a large family of transcriptional regulators found in many bact

SUBMITTER: Schuller A 

PROVIDER: S-EPMC3312330 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature