Epigenetic alteration of Wnt pathway antagonists in progressive glandular neoplasia of the lung.

ABSTRACT:

Background

Atypical adenomatous hyperplasia (AAH) is now recognized as a precursor lesion from which lung adenocarcinomas arise and thus represents an ideal target for studying the early genetic and epigenetic alterations associated with lung tumorigenesis such as alterations of the Wnt pathway.

Methods

We assessed the level of Wnt signaling activity in lung cancer cell lines by determining the level of active beta-catenin and determined the level of expression of Wnt antagonists APC, DKK1, DKK3, LKB1, SFRP1, 2, 4, 5, WIF1 and RUNX3 using reverse transcription-polymerase chain reaction. Using multiplex nested methylation-specific polymerase chain reaction, we analyzed promoter region methylation of these genes in resected lung tissue in the histopathologic sequence of glandu

SUBMITTER: Licchesi JD 

PROVIDER: S-EPMC3312609 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature