Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes.

ABSTRACT:

Background

Massively parallel sequencing technology is revolutionizing approaches to genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation Sequencing (NGS) has rapidly improved. In spite of this success, sequencing genomes or genomic regions with extremely biased base composition is still a great challenge to the currently available NGS platforms. The genomes of some important pathogenic organisms like Plasmodium falciparum (high AT content) and Mycobacterium tuberculosis (high GC content) display extremes of base composition. The standard library preparation procedures that employ PCR amplification have been shown to cause uneven read coverage particularly across AT and GC rich regions, leading to problems in genome assembly and variation anal

SUBMITTER: Oyola SO 

PROVIDER: S-EPMC3312816 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature