Opposing roles of σB and σB-controlled SpoVG in the global regulation of esxA in Staphylococcus aureus.

ABSTRACT:

Background

The production of virulence factors in Staphylococcus aureus is tightly controlled by a complex web of interacting regulators. EsxA is one of the virulence factors that are excreted by the specialized, type VII-like Ess secretion system of S. aureus. The esxA gene is part of the σB-dependent SpoVG subregulon. However, the mode of action of SpoVG and its impact on other global regulators acting on esxA transcription is as yet unknown.

Results

We demonstrate that the transcription of esxA is controlled by a regulatory cascade involving downstream σB-dependent regulatory elements, including the staphylococcal accessory regulator SarA, the ArlRS two-component system and SpoVG. The esxA gene, preceding the ess gene cluster, was shown to form a monocistronic transcript

SUBMITTER: Schulthess B 

PROVIDER: S-EPMC3313859 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature