A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.

ABSTRACT:

Background

The experimental murine model of leishmaniasis has been widely used to characterize the immune response against Leishmania. CBA mice develop severe lesions, while C57BL/6 present small chronic lesions under L. amazonensis infection. Employing a transcriptomic approach combined with biological network analysis, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite.

Results

The genes expressed by C57BL/6 and CBA macrophages, before and after infection, differ greatly, both with respect to absolute number as well as cell function. Uninfected C57BL/6 macrophages express genes involved in the deactivatio

SUBMITTER: Probst CM 

PROVIDER: S-EPMC3313874 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature