Cocaine hydrolase encoded in viral vector blocks the reinstatement of cocaine seeking in rats for 6 months.

ABSTRACT:

Background

Cocaine dependence is a pervasive disorder with high rates of relapse. In a previous study, direct administration of a quadruple mutant albumin-fused butyrylcholinesterase that efficiently catalyzes hydrolysis of cocaine to benzoic acid and ecgonine methyl ester acutely blocked cocaine seeking in an animal model of relapse. In the present experiments, these results were extended to achieve a long-duration blockade of cocaine seeking with a gene transfer paradigm using a related butyrylcholinesterase-based cocaine hydrolase (CocH).

Methods

Male and female rats were allowed to self-administer cocaine under a fixed-ratio 1 schedule of reinforcement for approximately 14 days. Following the final self-administration session, rats were injected with CocH vector or a con

SUBMITTER: Anker JJ 

PROVIDER: S-EPMC3314110 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature