Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).

ABSTRACT: The selective isolation of a sub-population of cells from a larger, mixed population is a critical preparatory process to many biomedical assays. Here, we present a new cell isolation platform with a unique set of advantages over existing devices. Our technology, termed Immiscible Filtration Assisted by Surface Tension, exploits physical phenomena associated with the microscale to establish fluidic barriers composed of immiscible liquids. By attaching magnetically-responsive particles to a target cell population via immunocapture, we can selectively transport this population across the immiscible barrier and into a separate aqueous solution. The high interfacial energy associated with the immiscible phase / aqueous phase boundaries prevents unwanted cells or other contaminants from inadver

SUBMITTER: Berry SM 

PROVIDER: S-EPMC3314424 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature