Characterization of an inducible promoter in different DNA copy number conditions.

ABSTRACT:

Background

The bottom-up programming of living organisms to implement novel user-defined biological capabilities is one of the main goals of synthetic biology. Currently, a predominant problem connected with the construction of even simple synthetic biological systems is the unpredictability of the genetic circuitry when assembled and incorporated in living cells. Copy number, transcriptional/translational demand and toxicity of the DNA-encoded functions are some of the major factors which may lead to cell overburdening and thus to nonlinear effects on system output. It is important to disclose the linearity working boundaries of engineered biological systems when dealing with such phenomena.

Results

The output of an N-3-oxohexanoyl-L-homoserine lactone (HSL)-inducible RFP-e

SUBMITTER: Zucca S 

PROVIDER: S-EPMC3314568 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature