Exploring the switchgrass transcriptome using second-generation sequencing technology.

ABSTRACT:

Background

Switchgrass (Panicum virgatum L.) is a C4 perennial grass and widely popular as an important bioenergy crop. To accelerate the pace of developing high yielding switchgrass cultivars adapted to diverse environmental niches, the generation of genomic resources for this plant is necessary. The large genome size and polyploid nature of switchgrass makes whole genome sequencing a daunting task even with current technologies. Exploring the transcriptional landscape using next generation sequencing technologies provides a viable alternative to whole genome sequencing in switchgrass.

Principal findings

Switchgrass cDNA libraries from germinating seedlings, emerging tillers, flowers, and dormant seeds were sequenced using Roche 454 GS-FLX Titanium technology, generating 98

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3315583 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature