Specific, simple and rapid detection of porcine circovirus type 2 using the loop-mediated isothermal amplification method.

ABSTRACT:

Background

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS), and porcine dermatitis and nephropathy syndrome (PDNS). It has caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS.

Results

A loop-mediated isothermal amplification (LAMP) assay was used to detect PCV2 in this study. Three pairs of primers were specially designed for recognizing eight distinct sequences of the ORF2 gene. This gene lies in the PCV2 virus genome sequence, and encodes the Rep protein that is involved in virus replication. Time and temperature conditions for amplification of PCV2 genes were optimized to be 55 min at 59°C. The analysis of clin

SUBMITTER: Zhao K 

PROVIDER: S-EPMC3315793 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature