Opposite GC skews at the 5' and 3' ends of genes in unicellular fungi.

ABSTRACT:

Background

GC-skews have previously been linked to transcription in some eukaryotes. They have been associated with transcription start sites, with the coding strand G-biased in mammals and C-biased in fungi and invertebrates.

Results

We show a consistent and highly significant pattern of GC-skew within genes of almost all unicellular fungi. The pattern of GC-skew is asymmetrical: the coding strand of genes is typically C-biased at the 5' ends but G-biased at the 3' ends, with intermediate skews at the middle of genes. Thus, the initiation, elongation, and termination phases of transcription are associated with different skews. This pattern influences the encoded proteins by generating differential usage of amino acids at the 5' and 3' ends of genes. These biases also affect

SUBMITTER: McLean MA 

PROVIDER: S-EPMC3315797 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature