Proteomic profiling of follicular and papillary thyroid tumors.

ABSTRACT:

Objective

Thyroid proteomics is a new direction in thyroid cancer research aiming at etiological understanding and biomarker identification for improved diagnosis.

Methods

Two-dimensional electrophoresis was applied to cytosolic protein extracts from frozen thyroid samples (ten follicular adenomas, nine follicular carcinomas, ten papillary carcinomas, and ten reference thyroids). Spots with differential expression were revealed by image and multivariate statistical analyses, and identified by mass spectrometry.

Results

A set of 25 protein spots significant for discriminating between the sample groups was identified. Proteins identified for nine of these spots were studied further including 14-3-3 protein beta/alpha, epsilon, and zeta/delta, peroxiredoxin 6, selenium-b

SUBMITTER: Sofiadis A 

PROVIDER: S-EPMC3315832 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature