The MicroArray Quality Control (MAQC)-II study of common practices for the development and validation of microarray-based predictive models.

ABSTRACT: Gene expression data from microarrays are being applied to predict preclinical and clinical endpoints, but the reliability of these predictions has not been established. In the MAQC-II project, 36 independent teams analyzed six microarray data sets to generate predictive models for classifying a sample with respect to one of 13 endpoints indicative of lung or liver toxicity in rodents, or of breast cancer, multiple myeloma or neuroblastoma in humans. In total, >30,000 models were built using many combinations of analytical methods. The teams generated predictive models without knowing the biological meaning of some of the endpoints and, to mimic clinical reality, tested the models on data that had not been used for training. We found that model performance depended largely on the endpoint

SUBMITTER: Shi L 

PROVIDER: S-EPMC3315840 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature