Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.

ABSTRACT: In higher eukaryotes many genes encode protein isoforms whose properties and biological roles are often poorly characterized. Here we describe systematic approaches for detection of either distinct isoforms, or separate pools of the same isoform, with differential biological properties. Using information from ion intensities we have estimated protein abundance levels and using rates of change in stable isotope labeling with amino acids in cell culture isotope ratios we measured turnover rates and subcellular distribution for the HeLa cell proteome. Protein isoforms were detected using three data analysis strategies that evaluate differences between stable isotope labeling with amino acids in cell culture isotope ratios for specific groups of peptides within the total set of peptides assign

SUBMITTER: Ahmad Y 

PROVIDER: S-EPMC3316725 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature