Molecular probe technology detects bacteria without culture.

ABSTRACT:

Background

Our ultimate goal is to detect the entire human microbiome, in health and in disease, in a single reaction tube, and employing only commercially available reagents. To that end, we adapted molecular inversion probes to detect bacteria using solely a massively multiplex molecular technology. This molecular probe technology does not require growth of the bacteria in culture. Rather, the molecular probe technology requires only a sequence of forty sequential bases unique to the genome of the bacterium of interest. In this communication, we report the first results of employing our molecular probes to detect bacteria in clinical samples.

Results

While the assay on Affymetrix GenFlex Tag16K arrays allows the multiplexing of the detection of the bacteria in each clinica

SUBMITTER: Hyman RW 

PROVIDER: S-EPMC3316761 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature